The cartoon has been widely criticised as "racist".
However, the executive chairman of News Corp Australia Michael Miller defended Knight, saying the criticism of his cartoon "shows the world has gone too PC".
After the match, Twitter users and tennis commentators alike said the penalties assessed to Williams were excessively harsh, and some said they may have reflected sexism and racism in tennis officiating.
Knight, like nearly every cartoonist, mocks the powerful and famous when required; whether it be prime ministers like Malcolm Turnbull or presidents like Donald Trump.
As noted by the Herald Sun, fellow cartoonist Michael Leunig said Knight's cartoon was not offensive, but truthful.
Herald Sun editor, Damon Johnson also chimed in.
The Herald Sun has defended its Serena Williams "angry baby" cartoon by republishing the drawing on the front page of its Wednesday edition. The 18-time Grand Slam victor said that "we can not measure ourselves by what we think we should also be able to get away with", adding, "In fact, this is the sort of behavior that no one should be engaging in on the court". He had given her a warning for coaching, then a point penalty for smashing her racket and, after she repeatedly expressed frustration, including calling him a "thief", Ramos levied the game penalty for verbal abuse.
Naomi Osaka, the young tennis star whose mortification across the net from her idol, Serena Williams, added to a memorable TV spectacle at the U.S. Open Saturday night, has rebounded to score a major endorsement deal.
Knight responded to a barrage of similar comments on his cartoon, saying it's not related to race or gender.
"A champion tennis player had a mega-tantrum on the world stage, and Mark's cartoon depicted that", he said.
"It rightly mocks poor behaviour by a tennis legend", Johnston tweeted.
In response, umpires are reportedly threatening to boycott Williams' matches, as they feel that Ramos is being unfairly criticized for his conduct during the match, according to The Times of London.
However, it was quickly slammed by critics around the world who compared the image to a Jim Crow-era representation of black women. Asked to address the disparity in Williams' fines and Roger Federer's $1,500 fine for an expletive-laden outburst during the 2009 U.S. Open men's final, Adams said the fines are up to the discretion of the grand slam administrator, not the USTA.
In a separate interview with the ABC, Knight said: 'I drew her as an African-American woman.
"You are a liar", Williams said, while pointing her finger.